Highly flexible copper tape decorated with Ag nanoarrays as ultrasensitive SERS platforms for multi-hazardous pollutant sensing.

A highly flexible and cost-effective copper tape decorated with silver nanoparticles (Cu-TAg) has been developed for surface-enhanced Raman spectroscopy (SERS) sensing of multi-hazardous environmental pollutants. Highly ordered and spherical-shaped silver nanoarrays have been fabricated using a low-cost thermal evaporation method. The structural, morphological, and optical properties of Cu-TAg sensors have been studied and correlated to the corresponding SERS performances. The size of nanoparticles has been successively tuned by varying the deposition time from 5 to 25 s. The nanoparticle sizes were enhanced with an increase in the evaporation time. SERS investigations have revealed that the sensing potential is subsequently improved with an increase in deposition time up to 10 s and then deteriorates with further increase in Ag deposition. The highest SERS activity was acquired for an optimum size of ~ 37 nm; further simulation studies confirmed this observation. Moreover, Cu-TAg sensors exhibited high sensitivity, reproducibility, and recycling characteristics to be used as excellent chemo-sensors. The lower detection limit estimation revealed that it can sense even in the pico-molar range for sensing of rhodamine 6G and methylene blue. The estimated enhancement factor of the sensor is found to be 9.4 × 107. Molecular-specific sensing of a wide range of pollutants such as rhodamine 6G, alizarin red, methylene blue, butylated hydroxy anisole, and penicillin-streptomycin is demonstrated with high efficiencies for micromolar spiked samples. Copper tape functionalized with Ag arrays thus demonstrated to be a promising candidate for low-cost and reusable chemo-sensors for environmental remediation applications.

Highly efficient, label free, ultrafast plasmonic SERS biosensor (silver nanoarrays/Si) to detect GJB2 gene expressed deafness mutations in real time validated with PCR studies.

Rapid diagnostics of any gene mutations related to organ loss is highly demanded now-a days to consume time as well to reduce cost. Currently, Surface enhanced Raman spectroscopy (SERS) is evolved to be a rapid investigating tool to screen gene mutations down to single molecule sensing with regard to the design and development of substrates used for sensing. The current research focuses on particular towards direct detection of deafness mutations associated with single and dual sites related to GJB2 gene. SERS Sensor construction is achieved with plasmonic silver nanoarrays on Si (SNA/Si) substrate by effortless wet chemical methods (Reaction time: 35 s; Concentration: 20 mM). The fabricated SNA/Si facilitates direct sensing of the deafness mutations of GJB2 gene in single as well dual sites with the enhancement of plasmonic hotspots. Normal DNA DMF-33 (GGGGGG) as well as Mutant DNA at single site DMF-9 (GGGGG) were validated by their guanine fingerprint Raman bands intensity quenching for mutant DNA DMF-9 at 1366 cm-1 and 1595 cm-1 respectively. Likewise, double mutations in DMF-19 are substitutional from G to A, portrayed highly intense fingerprint of Adenine Raman bands at 739 cm-1, 1432 cm-1, 1572 cm-1 in comparison to normal DNA (DMF-33). The findings were well analyzed with Raman mapping data which carries almost 625 scans for each DNA sample. The fabricated sensor exhibited the highest sensitivity towards DNA detection down to 0.1 pg/µL with utmost reproducibility. The current study aims to bring in creation of library files for deafness mutations to facilitate clinical diagnostics in a simple and rapid approach.

Smart optical sensing of multiple antibiotic residues from wastewater effluents with ensured specificity using SERS assisted with multivariate analysis.

Surface-enhanced Raman spectroscopy offers great potential for rapid and highly sensitive detection of pharmaceuticals from environmental sources. Herein, we investigated the feasibility of label-free sensing of antibiotic residues from wastewater effluents with high specificity by combining with multivariate analysis. Highly ordered silver nanoarrays with ∼34 nm roughness have been fabricated using a cost-effective electroless deposition technique. As-fabricated Ag arrays showed superior LSPR effects with an enhancement factor of 8 × 107. Excellent reproducibility has also been noticed with RSD values within 11%, whilst the sensor showed good stability and reusability characteristics for being used as a low-cost and reusable sensor. SERS studies demonstrated that antibiotics-spiked wastewater effluents can be detected with high efficiency in a label-free method. The molecular fingerprint bands of antibiotics such as sulfamethoxazole, sulfadiazine, and ciprofloxacin were well analyzed in effluent, tap, and deionized water. It has been found that antibiotics can be detected near picomolar levels; meanwhile, liquid chromatography-mass spectrometry (LC-MS) exhibited a detection limit within nanomolar concentrations only. Furthermore, the specificity of SERS sensing has been further analyzed using a multivariate analysis method, principal component analysis followed by linear discriminant analysis (PCA-LDA); which showed prominent discrimination to distinguish each antibiotic residue from wastewater effluents. The current study presented the potential of Ag nanoarray sensors for rapid, highly specific, and cost-effective analysis of pharmaceutical products for environmental remediation applications.

Multi-functional silver nanoprism-titanium dioxide hybrid nanoarrays for trace-level SERS sensing and photocatalytic removal of hazardous organic pollutants.

Owing to the excellent optoelectronic properties of metal nanoparticle-semiconductor interfaces; hybrid substrates with superior catalytic and sensing properties can be designed. In the present study, we have attempted to evaluate anisotropic silver nanoprisms (SNP) functionalized titanium dioxide (TiO2) particles for multifunctional applications such as SERS sensing and photocatalytic decomposition of hazardous organic pollutants. Hierarchical TiO2/SNP hybrid arrays have been fabricated via facile and low-cost casting techniques. The structural, compositional, and optical characteristics of TiO2/SNP hybrid arrays were well elucidated and correlated to SERS activities. SERS studies revealed that TiO2/SNP nanoarrays possess almost 288 times enhancement compared to bare TiO2 substrates and 2.6 times enhancement than pristine SNP. The fabricated nanoarrays demonstrated detection limits down to 10-12 M concentration levels and lower spot-to-spot variability of âˆ¼ 11%. The photocatalytic studies showed that almost 94 and 86% of rhodamine B and methylene blue were decomposed within 90 min of visible light exposure. Besides, two times enhancement in photocatalytic activities of TiO2/SNP hybrid substrates was also observed than bare TiO2. The highest photocatalytic activity was exhibited by SNP to TiO2 molar ratio of 1.5 × 10-3. The electrochemical surface area and the interfacial electron-transfer resistance were increased with the increment in TiO2/SNP composite load from 3 to 7 wt%. Differential Pulse Voltammetry (DPV) analysis revealed a higher RhB degradation potential of TiO2/SNP arrays than SNP or TiO2. The synthesized hybrids exhibited excellent reusability without any significant deterioration in photocatalytic properties over five successive cycles. TiO2/SNP hybrid arrays were proved to be multiple platforms for sensing and degrading hazardous pollutants for environmental applications.

Label-free DNA detection using silver nanoprism decorated silicon nanoparticles: Effect of silicon nanoparticle size and doping levels.

In the present work, we have fabricated silver nanoprism (AgNPrs)/silicon nanoparticle (SiNPs) hybrid arrays for highly sensitive detection of biomolecules via surface-enhanced Raman spectroscopy (SERS) technique. SiNPs having 7 to 37 nm in size and with phosphorous doping varying from 1 × 1019 to 1 × 1020 cm-3 were synthesized in nonthermal plasma synthesis. SiNPs were further immobilized on glass substrates using spin-coating, followed by deposition of AgNPrs using the drop-casting method. SERS studies showed that AgNPrs/SiNPs hybrid arrays exhibit substantial amplification of fingerprint bands of rhodamine 6G (R6G) compared to bare silicon as the reference. Raman signal intensity was found to be dependent on the size of SiNPs, with the largest nanoparticles exhibiting the highest SERS enhancement. In addition, an increase in phosphorous doping concentration was found to reduce R6G peak intensities. AgNPrs/SiNPs hybrid arrays showed excellent stability over time and high spot-to-spot reproducibility as well. Moreover, hybrid arrays enabled DNA detection through intense vibrational modes of human genomic DNA, with a lower detection limit of 1.5 pg/µL; indicating that AgNPrs/SiNPs sensors can serve as a reliable and cost-effective biosensing platform for rapid and label-free analysis of biomolecules.

Enhanced photocatalytic activities of silicon nanowires/graphene oxide nanocomposite: Effect of etching parameters.

Homogeneous and vertically aligned silicon nanowires (SiNWs) were successfully fabricated using silver assisted chemical etching technique. The prepared samples were characterized using scanning electron microscopy, transmission electron microscopy and atomic force microscopy. Photocatalytic degradation properties of graphene oxide (GO) modified SiNWs have been investigated. We found that the SiNWs morphology depends on etching time and etchant composition. The SiNWs length could be tuned from 1 to 42 µm, respectively when varying the etching time from 5 to 30 min. The etchant concentration was found to accelerate the etching process; doubling the concentrations increases the length of the SiNWs by a factor of two for fixed etching time. Changes in bundle morphology were also studied as function of etching parameters. The SiNWs diameter was found to be independent of etching time or etchant composition while the size of the SiNWs bundle increases with increasing etching time and etchant concentration. The addition of GO was found to improve significantly the photocatalytic activity of SiNWs. A strong correlation between etching parameters and photocatalysis efficiency has been observed, mainly for SiNWs prepared at optimum etching time and etchant concentrations of 10 min and 4:1:8. A degradation of 92% was obtained which further improved to 96% by addition of hydrogen peroxide. Only degradation efficiency of 16% and 31% has been observed for bare Si and GO/bare Si samples respectively. The obtained results demonstrate that the developed SiNWs/GO composite exhibits excellent photocatalytic performance and could be used as potential platform for the degradation of organic pollutants.

Structural effects of silver-nanoprism-decorated Si nanowires on surface-enhanced Raman scattering.

Surface enhanced Raman scattering (SERS) is an important analytical tool for the optochemical detection of molecules. The enhancement is commonly achieved by engineering (i) novel types and morphologies of plasmonic nanomaterials, and (ii) patterned or roughened supporting substrates of high surface area for increased light scattering and molecule adsorption. Si substrates can be easily and reproducibly textured for effective SERS applications. In this work, silver nanoprisms (AgNPr) coated silicon nanowire (SiNWs) of different morphologies have been prepared by metal-assisted chemical etching and tested for SERS detection of R6G dye. By varying the etching time from 5 to 30 min, the nanowires' lengths increased from 2.4 to 10.5 µm and resulted in a variable topological morphology of the substrates in terms of bundles and valleys. We found that an optimum of 10 min etching time led to the highest SERS enhancement of R6G on AgNPr/SiNWs at 612 cm-1 Raman shift (30× compared to R6G/Si and 2× compared to R6G/AgNPr/Si), with a detection limit comparable to that of state-of-the-art performances (down to 5×10-10 M of R6G). Such an enhancement is attributed to a middle ground between increased overall surface area of SiNWs, and the available bundle tops trapping the AgNPr and R6G molecules.

Relating pore size variation of poly (ɛ-caprolactone) scaffolds to molecular weight of porogen and evaluation of scaffold properties after degradation.

The major challenge in designing a scaffold for fabricating tissue engineered blood vessels is optimization of its microstructure for supporting uniform cellular in-growth with good mechanical integrity and degradation kinetics suitable for long-term implantation. In this study, we have investigated the feasibility of varying the pore size of poly(ɛ-caprolactone) (PCL) scaffold by altering the molecular weight of porogen and studied the effect of degradation on morphological characteristics and mechanical properties of scaffolds by correlating to the extent of degradation. Scaffolds with two different pore sizes were prepared by solvent casting and particulate leaching where poly(ethylene glycol) (PEG) porogens having two molecular weights (3400 and 8000) were used and subjected to in vitro degradation in phosphate buffered saline (PBS) upto six months. Microcomputed tomography studies of scaffolds revealed narrower pore size distribution when PEG-3400 was used as porogen and had 78% pores in the 12-24 µ range, whereas incorporation of PEG-8000 resulted in broader distribution with only 65% pores in the same range. Degradation resulted in scaffolds with narrower pore size distribution to have better retention of morphological and mechanical characteristics compared to scaffolds with broader distribution. Gravimetric and molecular weight studies also showed that scaffold degradation in both cases was only in initial stages after 6 months and PCL scaffolds had potential to be recommended for vascular tissue engineering applications.